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Biocatalyst Kits

The reactions catalyzed by enzymes have several advantages over those traditional chemical transformations, such as mild reaction conditions, high specificity (selectivity), low cost and environmental compatibility. The judicial selection and use of an enzyme for a reaction can be greatly facilitated by performing a high throughput enzymatic reaction screening with enzyme screening kits. We offer 15 enzyme screening kits targeting different classes of enzymatic transformations.

1. KRED Kit

It contains 120 ketoreductases (KREDs), and is a useful tool to quickly determine the feasibility of using a KRED for a regio- and stereoselective reduction of a wide range of aldehydes, ß-ketoesters, a-ketoesters and ketones

Reaction Type
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+/NAD+, 0.4 mg D-glucose, 0.2 mg glucose dehydrogenase.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+/NAD+, 0.4 mg D-glucose, 0.2 mg glucose dehydrogenase.
  3. Co-enzyme system for Column 1-9 is NADP+, for Column 10-12 is NAD+.
2. ATA Kit

It contains 20 ?-transaminases (ATAs), and is a useful tool to catalyze regio- and stereoselective synthesis of chiral amines, amino acids and their derivatives from a variety of aliphatic and aromatic ketoacids, aldehydes, ketones and ketoses.

Reaction Type
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.0005 mg pyridoxal-5–phosphate, 0.121 mg phenylethanamine.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.0005 mg pyridoxal-5–phosphate, 0.121 mg phenylethanamine.
  3. D3 contains 1 mg enzyme, 1.07 mg K2HPO4,0.52 mg KH2PO4,0.02 mg NAD+,0.12 mg ammonium formate, 0.2 mg formate dehydrogenase.
3. ERED Kit

It contains 23 ene reductases (EREDs), and is a useful tool to catalyze regio- and stereoselective double bond reduction of a, ß-unsaturated aldehydes, ketones, nitriles, nitros, and amides.

Reaction Type
EWG = aldehydes, ketones, nitros, nitriles.
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+/NAD+, 0.4 mg D-glucose, 0.2 mg glucose dehydrogenase.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+/NAD+, 0.4 mg D-glucose, 0.2 mg glucose dehydrogenase.
  3. Co-enzyme system for Column 1-3 is NADP, for Column 4 is NAD+.
4. DERA Kit

It contains 6 aldolases (DERAs), and is a useful tool to catalyzes enantioselective carbon-carbon bond formation, generating up to two chiral centers under mild reaction conditions. They are emerging as powerful and cost efficient tools for the industrial synthesis of chiral molecules.

Reaction Type
  1. The kit contains 94 x 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4.
5. AMD Kit

It contains 16 Amidases (AMDs), and is a useful tool to catalyze regio- and stereoselective synthesis of chiral carboxylic acids and their derivatives from a variety of aliphatic and aromatic amides.

Reaction Type
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4.
6. NHT Kit

It contains 24 nitrilases (NHTs), and is a useful tool to catalyze the synthesis of chiral amides through regio- and stereoselective hydrolysis of a variety of aliphatic and aromatic nitriles.

Reaction Type
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4.
7. NIT Kit

It contains 40 nitrilases (NITs), and is a useful tool to catalyze the synthesis of chiral carboxylic acids through regio- and stereoselective hydrolysis of a wide range of aliphatic and aromatic nitriles.

Reaction Type
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4.
8. D-AADH Kit

It contains 7 D-amino acid dehydrogenases (D-AADHs), and is a useful tool to catalyze regio- and stereoselective synthesis of D-amino acids from corresponding 2-keto acids with ammonia.

Reaction Type
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+, 0.4 mg D-glucose, 0.2 mg glucose dehydrogenase.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+, 0.4 mg D-glucose, 0.2 mg glucose dehydrogenase.
9. HNL Kit

It contains 29 nitrilases (HNLs), and is a useful tool to regio- and stereo- selective synthesis of (R)-cyanohydrins or (S)-cyanohydrins from a variety of aromatic, aliphatic and heterocyclic aldehydes or even ketones.

Reaction Type
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4.
  3. Stereoselective synthesis for Column 1-3 is S, for Column 4-5 is R.
10. AOX Kit

It contains 5 alcohol oxidases (AOXs), and is a useful tool to catalyze oxidation of fatty alcohols or aryl-alcohols to form aldehydes or aldehydes.

Reaction Type
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4.
11. EH Kit

It contains 2 epoxide hydrolases (EHs), and is a useful tool to catalyze the synthesis of chiral alcohols through stereoselective hydrolysis of aliphatic and aromatic epoxides.

Reaction Type
  1. The kit contains 94 x 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4.
12. FDH Kit

It contains 2 formate dehydrogenases (FDHs), and is a used for recycling NADH with ammonium formate as the reducing agent.

Reaction Type
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+/NAD+, 0.4 mg ammonium formate.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+/NAD+, 0.4 mg ammonium formate.
13. GDH Kit

It contains 4 glucose dehydrogenases (GDHs), and is a used for recycling NADH and NADPH with glucose as the reducing agent.

Reaction Type
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+/NAD+, 0.4 mg D-glucose.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+/NAD+, 0.4 mg D-glucose.
14. CYP Kit

It contains 5 cytochrome P450 monooxygenases (CYPs), and is a useful tool to hydroxylate unreactive carbons (C-H activation) or oxidize heteroatoms (N, S, etc.) in the presence of oxygen.

Reaction Type
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+, 0.4 mg D-glucose, 0.2 mg glucose dehydrogenase.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+, 0.4 mg D-glucose, 0.2 mg glucose dehydrogenase.
15. NTR Kit

It contains 12 nitro reductases (NTRs), and is a useful tool to catalyze the synthesis of amines from corresponding nitro compounds.

Reaction Type
  1. The kit contains 1 mg enzyme, 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+/NAD+, 0.4 mg D-glucose, 0.2 mg glucose dehydrogenase.
  2. Control-1 and Control-2 contains 1.07 mg K2HPO4, 0.52 mg KH2PO4, 0.02 mg NADP+/NAD+, 0.4 mg D-glucose, 0.2 mg glucose dehydrogenase.
  3. Co-enzyme system for A1 is NAD+, for the kit is NADP+.

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